| 1. Round | 10/08/2024 | 1. FC Slovácko [Youth B] | - | FK Mladá Boleslav [Youth B] | 0:2 |  |
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| 2. Round | 17/08/2024 | FK Pardubice [Youth B] | - | 1. FC Slovácko [Youth B] | 2:3 | ||||
| 3. Round | 24/08/2024 | 1. FC Slovácko [Youth B] | - | Slavia Praha [Youth B] | 2:4 | ||||
| 4. Round | 29/08/2024 | FK Jablonec [Youth B] | - | 1. FC Slovácko [Youth B] | 2:6 | ||||
| 5. Round | 07/09/2024 | 1. FC Slovácko [Youth B] | - | Slovan Liberec [Youth B] | 2:1 | ||||
| 10. Round | 12/10/2024 | 1. FC Slovácko [Youth B] | - | FC Zbrojovka Brno [Youth B] | 3:1 | ||||
| 11. Round | 19/10/2024 | Banik Ostrava [Youth B] | - | 1. FC Slovácko [Youth B] | 7:5 | ||||
| 12. Round | 26/10/2024 | 1. FC Slovácko [Youth B] | - | Dynamo České Budějovice [Youth B] | 2:1 | ||||
| 14. Round | 09/11/2024 | 1. FC Slovácko [Youth B] | - | Viktoria Plzeň [Youth B] | 0:1 | ||||
| 15. Round | 16/11/2024 | Vysočina Jihlava [Youth B] | - | 1. FC Slovácko [Youth B] | 4:3 |
Czech Republic