| 20. Round | 05/04/2025 | Slovan Liberec [Youth B] | - | 1. FC Slovácko [Youth B] | 3:5 | ||||
| 21. Round | 12/04/2025 | 1. FC Slovácko [Youth B] | - | SFC Opava [Youth B] | 3:0 | ||||
| 23. Round | 26/04/2025 | FC Zlín [Youth B] | - | 1. FC Slovácko [Youth B] | 2:3 | ||||
| 25. Round | 10/05/2025 | FC Zbrojovka Brno [Youth B] | - | 1. FC Slovácko [Youth B] | 2:1 | ||||
| 26. Round | 13/05/2025 | 1. FC Slovácko [Youth B] | - | Banik Ostrava [Youth B] | 2:2 | ||||
| 28. Round | 31/05/2025 | 1. FC Slovácko [Youth B] | - | FK Dukla Praha [Youth B] | 3:4 |
Czech Republic