| 17. Round | 11/03/2023 | FK Pardubice [Youth] | - | 1. FC Slovácko [Youth] | 1:2 | ||||
| 19. Round | 25/03/2023 | Dynamo České Budějovice [Youth] | - | 1. FC Slovácko [Youth] | 2:1 |  |
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| 20. Round | 01/04/2023 | 1. FC Slovácko [Youth] | - | Sigma Olomouc [Youth] | 0:1 | ||||
| 22. Round | 15/04/2023 | FK Mladá Boleslav [Youth] | - | 1. FC Slovácko [Youth] | 1:1 | ||||
| 27. Round | 20/05/2023 | 1. FC Slovácko [Youth] | - | MFK Karviná [Youth] | 1:0 | ||||
| 28. Round | 27/05/2023 | FK Příbram [Youth] | - | 1. FC Slovácko [Youth] | 0:8 |
Czech Republic