| 1. Round | 10/08/2024 | Banik Ostrava [Youth B] | - | Dynamo České Budějovice [Youth B] | 3:1 | ||||
| 2. Round | 17/08/2024 | FK Dukla Praha [Youth B] | - | Banik Ostrava [Youth B] | 0:1 | ||||
| 3. Round | 28/08/2024 | Banik Ostrava [Youth B] | - | Viktoria Plzeň [Youth B] | 3:3 | ||||
| 4. Round | 31/08/2024 | Vysočina Jihlava [Youth B] | - | Banik Ostrava [Youth B] | 1:5 |  |
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| 7. Round | 21/09/2024 | Banik Ostrava [Youth B] | - | Slavia Praha [Youth B] | 1:5 | ||||
| 8. Round | 28/09/2024 | FK Jablonec [Youth B] | - | Banik Ostrava [Youth B] | 1:6 | |
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| 11. Round | 19/10/2024 | Banik Ostrava [Youth B] | - | 1. FC Slovácko [Youth B] | 7:5 |  |
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| 5. Round | 23/10/2024 | Banik Ostrava [Youth B] | - | FK Mladá Boleslav [Youth B] | 4:2 | ||||
| 12. Round | 29/10/2024 | FC Zlín [Youth B] | - | Banik Ostrava [Youth B] | 1:4 | ||||
| 13. Round | 02/11/2024 | Banik Ostrava [Youth B] | - | Sigma Olomouc [Youth B] | 1:0 | |
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| 6. Round | 06/11/2024 | FK Pardubice [Youth B] | - | Banik Ostrava [Youth B] | 1:1 | ||||
| 14. Round | 09/11/2024 | FC Zbrojovka Brno [Youth B] | - | Banik Ostrava [Youth B] | 3:4 | ||||
| 15. Round | 16/11/2024 | Banik Ostrava [Youth B] | - | AC Sparta Praha [Youth B] | 1:4 |
Czech Republic