| 1. Round | 24/07/2023 | Zbrojovka Brno | - | FC Sellier & Bellot Vlašim | 1:1 | ||||
| 2. Round | 30/07/2023 | Sigma Olomouc B | - | Zbrojovka Brno | 1:2 | ||||
| 3. Round | 02/08/2023 | Zbrojovka Brno | - | FK Dukla Praha | 0:2 | ||||
| 4. Round | 05/08/2023 | FK MAS Táborsko | - | Zbrojovka Brno | 2:1 | ||||
| 5. Round | 12/08/2023 | Zbrojovka Brno | - | MFK Chrudim | 4:1 | ||||
| 6. Round | 18/08/2023 | AC Sparta Praha B | - | Zbrojovka Brno | 1:2 | ||||
| 7. Round | 26/08/2023 | 1. SK Prostějov | - | Zbrojovka Brno | 0:1 | ||||
| 8. Round | 02/09/2023 | Zbrojovka Brno | - | FK Varnsdorf | 1:0 | ||||
| 9. Round | 15/09/2023 | Vysočina Jihlava | - | Zbrojovka Brno | 0:1 | ||||
| 10. Round | 23/09/2023 | Zbrojovka Brno | - | MFK Vyškov | 0:1 | ||||
| 11. Round | 30/09/2023 | SFC Opava | - | Zbrojovka Brno | 2:1 |  |
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| 13. Round | 22/10/2023 | Viktoria Žižkov | - | Zbrojovka Brno | 4:2 | ||||
| 14. Round | 27/10/2023 | Zbrojovka Brno | - | SK Líšeň | 3:0 |  |
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| 15. Round | 04/11/2023 | Hanácká Slávia Kroměříž | - | Zbrojovka Brno | 0:3 | ||||
| 16. Round | 11/11/2023 | Zbrojovka Brno | - | Sigma Olomouc B | 0:1 | ||||
| 17. Round | 02/03/2024 | FK Dukla Praha | - | Zbrojovka Brno | 3:3 | ||||
| 30. Round | 25/05/2024 | FC Sellier & Bellot Vlašim | - | Zbrojovka Brno | 2:2 |
Czech Republic