| 32. Round | 02/03/2025 | FK Jablonec B | - | FK Mlada Boleslav B | 0:2 | ||||
| 18. Round | 09/03/2025 | FK Mlada Boleslav B | - | FK Pardubice B | 1:0 | ||||
| 23. Round | 13/04/2025 | FK Teplice B | - | FK Mlada Boleslav B | 1:3 | ||||
| 24. Round | 18/04/2025 | FK Mlada Boleslav B | - | Jiskra Ústí nad Orlicí | 0:0 |  |
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| 26. Round | 04/05/2025 | SK Zápy | - | FK Mlada Boleslav B | 2:2 | |
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| 33. Round | 07/05/2025 | FK Mlada Boleslav B | - | FC Hradec Králové B | 1:4 | ||||
| 27. Round | 10/05/2025 | FK Mlada Boleslav B | - | FK Ústí nad Labem | 0:3 | ||||
| 28. Round | 17/05/2025 | FK Baník Most-Souš | - | FK Mlada Boleslav B | 2:2 | |
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| 29. Round | 24/05/2025 | FK Mlada Boleslav B | - | Chlumec nad Cidlinou | 1:3 | ||||
| 30. Round | 31/05/2025 | Sokol Živanice | - | FK Mlada Boleslav B | 3:1 |
Czech Republic