| 5. Round | 01/09/2024 | 1. FC Slovácko B | - | TJ Start Brno | 3:2 |  |
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| 6. Round | 07/09/2024 | TJ Start Brno | - | FK Frýdek-Místek | 2:1 | ||||
| 17. Round | 11/09/2024 | TJ Start Brno | - | FC Strání | 2:0 | ||||
| 8. Round | 22/09/2024 | 1. SC Znojmo | - | TJ Start Brno | 2:3 | ||||
| 9. Round | 27/09/2024 | TJ Start Brno | - | FC Hlučín | 0:0 | ||||
| 7. Round | 01/10/2024 | FC Slovan Rosice | - | TJ Start Brno | 0:0 | |
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| 10. Round | 06/10/2024 | MFK Karviná B | - | TJ Start Brno | 5:2 | ||||
| 11. Round | 12/10/2024 | TJ Start Brno | - | FC Zlín B | 0:0 | ||||
| 12. Round | 20/10/2024 | FC Zbrojovka Brno B | - | TJ Start Brno | 1:2 | ||||
| 13. Round | 27/10/2024 | TJ Start Brno | - | FK Blansko | 2:2 | ||||
| 14. Round | 03/11/2024 | ČSK Uherský Brod | - | TJ Start Brno | 2:2 | |
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| 15. Round | 10/11/2024 | TJ Start Brno | - | FC Zlínsko | 1:1 | ||||
| 16. Round | 16/11/2024 | SK Uničov | - | TJ Start Brno | 3:0 | ||||
| 18. Round | 09/03/2025 | TJ Start Brno | - | Hanácká Slávia Kroměříž | 0:1 | ||||
| 20. Round | 22/03/2025 | TJ Start Brno | - | FK Třinec | 2:1 | ||||
| 19. Round | 26/03/2025 | TJ Unie Hlubina | - | TJ Start Brno | 4:1 | |
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| 21. Round | 29/03/2025 | FK Hodonín | - | TJ Start Brno | 2:0 | ||||
| 22. Round | 05/04/2025 | TJ Start Brno | - | 1. FC Slovácko B | 0:1 |
Czech Republic