| 1. Round | 30/07/2000 | CU Bohemians Praha | - | FK Jablonec 97 | 1:1 | ||||
| 2. Round | 06/08/2000 | Dynamo České Budějovice | - | CU Bohemians Praha | 0:1 | ||||
| 5. Round | 27/08/2000 | CU Bohemians Praha | - | 1. FC Synot Stare Mesto | 1:1 | ||||
| 6. Round | 10/09/2000 | Viktoria Plzeň | - | CU Bohemians Praha | 1:1 | ||||
| 7. Round | 15/09/2000 | CU Bohemians Praha | - | Chmel Blsany | 4:0 |  |
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| 8. Round | 23/09/2000 | CU Bohemians Praha | - | Slovan Liberec | 0:0 | ||||
| 10. Round | 14/10/2000 | CU Bohemians Praha | - | AC Sparta Praha | 4:2 | ||||
| 11. Round | 22/10/2000 | FK Teplice | - | CU Bohemians Praha | 3:0 | |
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| 12. Round | 27/10/2000 | CU Bohemians Praha | - | Sigma Olomouc | 2:2 | ||||
| 13. Round | 05/11/2000 | FC Marila Příbram | - | CU Bohemians Praha | 3:0 | |
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| 15. Round | 19/11/2000 | Viktoria Žižkov | - | CU Bohemians Praha | 2:1 | |
Czech Republic