| 1. Round | 09/08/2024 | FC Zlín [Youth] | - | Vysočina Jihlava [Youth] | 3:1 | ||||
| 2. Round | 16/08/2024 | Vysočina Jihlava [Youth] | - | Sigma Olomouc [Youth] | 2:5 | ||||
| 5. Round | 20/08/2024 | Dynamo České Budějovice [Youth] | - | Vysočina Jihlava [Youth] | 3:1 | ||||
| 12. Round | 26/10/2024 | FK Jablonec [Youth] | - | Vysočina Jihlava [Youth] | 0:1 | ||||
| 14. Round | 09/11/2024 | SFC Opava [Youth] | - | Vysočina Jihlava [Youth] | 2:2 | ||||
| 15. Round | 16/11/2024 | Vysočina Jihlava [Youth] | - | 1. FC Slovácko [Youth] | 1:1 | ||||
| 21. Round | 12/04/2025 | FK Dukla Praha [Youth] | - | Vysočina Jihlava [Youth] | 2:1 | ||||
| 23. Round | 26/04/2025 | Vysočina Jihlava [Youth] | - | AC Sparta Praha [Youth] | 1:0 | ||||
| 26. Round | 17/05/2025 | Slavia Praha [Youth] | - | Vysočina Jihlava [Youth] | 2:2 | ||||
| 27. Round | 23/05/2025 | Vysočina Jihlava [Youth] | - | FK Jablonec [Youth] | 2:0 | ||||
| 28. Round | 31/05/2025 | Slovan Liberec [Youth] | - | Vysočina Jihlava [Youth] | 2:2 | ||||
| 29. Round | 07/06/2025 | Vysočina Jihlava [Youth] | - | SFC Opava [Youth] | 3:1 | ||||
| 30. Round | 11/06/2025 | 1. FC Slovácko [Youth] | - | Vysočina Jihlava [Youth] | 3:3 |  |
Czech Republic