| 1. Round | 04/08/2024 | SK Uničov | - | MFK Karviná B | 2:1 | ||||
| 2. Round | 11/08/2024 | MFK Karviná B | - | FC Strání | 6:1 |  |
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| 4. Round | 25/08/2024 | MFK Karviná B | - | TJ Unie Hlubina | 4:0 | |
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| 5. Round | 30/08/2024 | FK Třinec | - | MFK Karviná B | 5:1 | ||||
| 6. Round | 08/09/2024 | MFK Karviná B | - | FK Hodonín | 1:2 | ||||
| 17. Round | 11/09/2024 | MFK Karviná B | - | FC Zlínsko | 2:0 |  |
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| 8. Round | 22/09/2024 | MFK Karviná B | - | FK Frýdek-Místek | 0:1 | ||||
| 9. Round | 28/09/2024 | FC Slovan Rosice | - | MFK Karviná B | 6:3 | |
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| 3. Round | 02/10/2024 | Hanácká Slávia Kroměříž | - | MFK Karviná B | 3:2 | |
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| 10. Round | 06/10/2024 | MFK Karviná B | - | TJ Start Brno | 5:2 | ||||
| 11. Round | 11/10/2024 | FC Hlučín | - | MFK Karviná B | 3:0 | |
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| 13. Round | 27/10/2024 | MFK Karviná B | - | FC Zlín B | 1:2 | ||||
| 14. Round | 03/11/2024 | FC Zbrojovka Brno B | - | MFK Karviná B | 3:0 | ||||
| 7. Round | 06/11/2024 | 1. FC Slovácko B | - | MFK Karviná B | 1:2 | ||||
| 15. Round | 10/11/2024 | MFK Karviná B | - | FK Blansko | 1:2 | ||||
| 16. Round | 16/11/2024 | ČSK Uherský Brod | - | MFK Karviná B | 2:0 |
Czech Republic