| 5. Round | 10/08/2024 | FK MAS Táborsko | - | Zbrojovka Brno | 0:1 |  |
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| 6. Round | 18/08/2024 | Zbrojovka Brno | - | AC Sparta Praha B | 0:0 | ||||
| 7. Round | 26/08/2024 | MFK Vyškov | - | Zbrojovka Brno | 1:0 | |
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| 8. Round | 01/09/2024 | Zbrojovka Brno | - | FC Zlín | 1:2 | ||||
| 10. Round | 21/09/2024 | Zbrojovka Brno | - | SK Líšeň | 1:1 | ||||
| 11. Round | 29/09/2024 | Sigma Olomouc B | - | Zbrojovka Brno | 4:1 | ||||
| 9. Round | 02/10/2024 | 1. SK Prostějov | - | Zbrojovka Brno | 3:1 | ||||
| 13. Round | 20/10/2024 | Viktoria Žižkov | - | Zbrojovka Brno | 4:2 | ||||
| 15. Round | 03/11/2024 | Baník Ostrava B | - | Zbrojovka Brno | 1:2 | ||||
| 16. Round | 09/11/2024 | Zbrojovka Brno | - | SFC Opava | 2:2 |
Germany