| 1. Round | 09/08/2024 | FC Chomutov | - | Bohemians Praha 1905 B | 3:3 |  |
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| 2. Round | 17/08/2024 | SK Petřín Plzeň | - | FC Chomutov | 1:1 | |
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| 3. Round | 23/08/2024 | FC Chomutov | - | FK MAS Táborsko B | 1:2 | ||||
| 17. Round | 28/08/2024 | FK Motorlet Praha | - | FC Chomutov | 1:1 | ||||
| 4. Round | 01/09/2024 | FC Písek | - | FC Chomutov | 2:0 | ||||
| 5. Round | 06/09/2024 | FC Chomutov | - | FK Dukla Praha B | 3:4 | |
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| 7. Round | 20/09/2024 | FC Chomutov | - | Sokol Hostouň | 0:2 | |
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| 8. Round | 29/09/2024 | Viktoria Plzeň B | - | FC Chomutov | 4:1 | |
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| 9. Round | 05/10/2024 | FC Chomutov | - | FK Admira Praha | 3:0 | |
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| 10. Round | 12/10/2024 | FK Loko Vltavín | - | FC Chomutov | 1:1 | |
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| 11. Round | 19/10/2024 | FC Chomutov | - | TJ Jiskra Domažlice | 1:2 | |
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| 12. Round | 27/10/2024 | Povltavská FA | - | FC Chomutov | 2:2 | |
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| 13. Round | 01/11/2024 | FC Chomutov | - | Dynamo České Budějovice B | 1:0 | |
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| 15. Round | 15/11/2024 | FK Příbram | - | FC Chomutov | 1:0 | |
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| 16. Round | 23/11/2024 | FC Chomutov | - | Slovan Velvary | 3:2 | |
Czech Republic
Ukraine