| 1. Round | 27/07/2009 | Bohemians 1905 | - | 1. FC Brno | 0:0 | ||||
| 2. Round | 31/07/2009 | AC Sparta Praha | - | Bohemians 1905 | 0:0 | ||||
| 3. Round | 09/08/2009 | Bohemians 1905 | - | FK Baumit Jablonec | 0:1 | ||||
| 4. Round | 16/08/2009 | Sigma Olomouc | - | Bohemians 1905 | 1:1 | ||||
| 5. Round | 23/08/2009 | Bohemians 1905 | - | Viktoria Plzeň | 0:1 |  |
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| 6. Round | 29/08/2009 | FK Mladá Boleslav | - | Bohemians 1905 | 0:1 | ||||
| 11. Round | 18/10/2009 | Bohemians 1905 | - | 1. FC Slovácko | 0:1 | ||||
| 12. Round | 24/10/2009 | Baník Ostrava | - | Bohemians 1905 | 3:1 | ||||
| 15. Round | 21/11/2009 | SK Kladno | - | Bohemians 1905 | 1:2 | ||||
| 16. Round | 28/11/2009 | Bohemians 1905 | - | AC Sparta Praha | 0:2 | ||||
| 17. Round | 28/02/2010 | FK Baumit Jablonec | - | Bohemians 1905 | 3:0 | ||||
| 18. Round | 06/03/2010 | Bohemians 1905 | - | Sigma Olomouc | 2:0 | ||||
| 19. Round | 14/03/2010 | Viktoria Plzeň | - | Bohemians 1905 | 3:0 | ||||
| 20. Round | 21/03/2010 | Bohemians 1905 | - | FK Mladá Boleslav | 2:0 | ||||
| 23. Round | 05/04/2010 | Slavia Praha | - | Bohemians 1905 | 3:0 | ||||
| 30. Round | 15/05/2010 | 1. FC Brno | - | Bohemians 1905 | 1:2 |
Czech Republic