| 10. Round | 21/09/2024 | Zbrojovka Brno | - | SK Líšeň | 1:1 | ||||
| 11. Round | 29/09/2024 | Sigma Olomouc B | - | Zbrojovka Brno | 4:1 | ||||
| 9. Round | 02/10/2024 | 1. SK Prostějov | - | Zbrojovka Brno | 3:1 | ||||
| 12. Round | 05/10/2024 | Zbrojovka Brno | - | FC Sellier & Bellot Vlašim | 2:2 | ||||
| 13. Round | 20/10/2024 | Viktoria Žižkov | - | Zbrojovka Brno | 4:2 | ||||
| 14. Round | 25/10/2024 | Zbrojovka Brno | - | Slavia Praha B | 1:1 | ||||
| 15. Round | 03/11/2024 | Baník Ostrava B | - | Zbrojovka Brno | 1:2 | ||||
| 16. Round | 09/11/2024 | Zbrojovka Brno | - | SFC Opava | 2:2 |
Czech Republic