| 1. Round | 21/07/2024 | FK Varnsdorf | - | Viktoria Žižkov | 1:1 | ||||
| 2. Round | 28/07/2024 | Viktoria Žižkov | - | SK Líšeň | 1:4 |  |
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| 3. Round | 31/07/2024 | Sigma Olomouc B | - | Viktoria Žižkov | 1:1 | ||||
| 4. Round | 04/08/2024 | Viktoria Žižkov | - | 1. SK Prostějov | 3:0 | ||||
| 5. Round | 09/08/2024 | FC Zlín | - | Viktoria Žižkov | 1:0 | ||||
| 6. Round | 18/08/2024 | Viktoria Žižkov | - | FK MAS Táborsko | 3:1 | ||||
| 7. Round | 25/08/2024 | Viktoria Žižkov | - | FC Sellier & Bellot Vlašim | 1:1 | ||||
| 8. Round | 30/08/2024 | Vysočina Jihlava | - | Viktoria Žižkov | 2:1 | ||||
| 11. Round | 28/09/2024 | Viktoria Žižkov | - | SFC Opava | 4:1 | ||||
| 9. Round | 02/10/2024 | Viktoria Žižkov | - | Baník Ostrava B | 0:1 | ||||
| 12. Round | 05/10/2024 | MFK Chrudim | - | Viktoria Žižkov | 1:5 | ||||
| 13. Round | 20/10/2024 | Viktoria Žižkov | - | Zbrojovka Brno | 4:2 | ||||
| 14. Round | 27/10/2024 | AC Sparta Praha B | - | Viktoria Žižkov | 1:2 | |
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| 15. Round | 03/11/2024 | Viktoria Žižkov | - | MFK Vyškov | 0:3 |
Czech Republic