| 21. Round | 30/03/2025 | FK Teplice B | - | FK Kolín | 2:2 | ||||
| 22. Round | 05/04/2025 | FK Kolín | - | FK Jablonec B | 2:1 | ||||
| 23. Round | 12/04/2025 | Jiskra Ústí nad Orlicí | - | FK Kolín | 2:1 | ||||
| 24. Round | 19/04/2025 | FK Kolín | - | FC Hradec Králové B | 0:0 | ||||
| 25. Round | 27/04/2025 | FK Mlada Boleslav B | - | FK Kolín | 1:0 | ||||
| 26. Round | 03/05/2025 | FK Kolín | - | Arsenal Česká Lípa | 1:1 | ||||
| 33. Round | 07/05/2025 | Chlumec nad Cidlinou | - | FK Kolín | 1:3 | ||||
| 27. Round | 11/05/2025 | SK Zápy | - | FK Kolín | 2:2 | ||||
| 28. Round | 17/05/2025 | FK Kolín | - | FK Pardubice B | 1:0 | ||||
| 34. Round | 21/05/2025 | FK Kolín | - | Sokol Brozany | 1:0 | ||||
| 29. Round | 24/05/2025 | FK Ústí nad Labem | - | FK Kolín | 3:1 |  |
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| 30. Round | 31/05/2025 | FK Kolín | - | Benátky nad Jizerou | 1:1 |
Czech Republic