| 1. Round | 10/08/2024 | FC Zbrojovka Brno [Youth B] | - | FK Dukla Praha [Youth B] | 4:1 | ||||
| 2. Round | 17/08/2024 | FK Dukla Praha [Youth B] | - | Banik Ostrava [Youth B] | 0:1 | ||||
| 3. Round | 24/08/2024 | Dynamo České Budějovice [Youth B] | - | FK Dukla Praha [Youth B] | 3:3 | ||||
| 5. Round | 07/09/2024 | FK Dukla Praha [Youth B] | - | Viktoria Plzeň [Youth B] | 1:2 | ||||
| 6. Round | 18/09/2024 | Vysočina Jihlava [Youth B] | - | FK Dukla Praha [Youth B] | 0:3 | ||||
| 7. Round | 21/09/2024 | FK Dukla Praha [Youth B] | - | FK Mladá Boleslav [Youth B] | 1:3 | ||||
| 12. Round | 26/10/2024 | SFC Opava [Youth B] | - | FK Dukla Praha [Youth B] | 3:2 |  |
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| 13. Round | 02/11/2024 | FK Dukla Praha [Youth B] | - | 1. FC Slovácko [Youth B] | 5:3 | ||||
| 14. Round | 09/11/2024 | FC Zlín [Youth B] | - | FK Dukla Praha [Youth B] | 1:2 |
Czech Republic