| 6. Round | 09/09/2023 | FK Kolín | - | Slovan Velvary | 0:4 | ||||
| 1. Round | 13/09/2023 | Sokol Živanice | - | FK Kolín | 3:0 |  |
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| 7. Round | 17/09/2023 | FK Mlada Boleslav B | - | FK Kolín | 1:2 | ||||
| 8. Round | 22/09/2023 | FK Kolín | - | FK Přepeře | 6:1 | ||||
| 9. Round | 01/10/2023 | FC Hradec Králové B | - | FK Kolín | 1:1 | ||||
| 10. Round | 07/10/2023 | FK Kolín | - | SK Zápy | 2:2 | |
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| 11. Round | 15/10/2023 | Slovan Liberec B | - | FK Kolín | 0:0 | ||||
| 13. Round | 29/10/2023 | Chlumec nad Cidlinou | - | FK Kolín | 3:3 | ||||
| 16. Round | 02/03/2024 | FK Kolín | - | Sokol Živanice | 1:4 | ||||
| 17. Round | 09/03/2024 | Arsenal Česká Lípa | - | FK Kolín | 1:1 | |
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| 18. Round | 16/03/2024 | FK Kolín | - | FK Baník Most-Souš | 2:1 | |
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| 20. Round | 29/03/2024 | FK Kolín | - | FK Jablonec B | 1:0 |  |
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| 21. Round | 06/04/2024 | Slovan Velvary | - | FK Kolín | 1:1 | |
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| 22. Round | 13/04/2024 | FK Kolín | - | FK Mlada Boleslav B | 1:0 | ||||
| 23. Round | 20/04/2024 | FK Přepeře | - | FK Kolín | 2:0 | ||||
| 25. Round | 05/05/2024 | SK Zápy | - | FK Kolín | 3:0 | ||||
| 26. Round | 11/05/2024 | FK Kolín | - | Slovan Liberec B | 5:0 | ||||
| 30. Round | 29/05/2024 | FK Kolín | - | Sokol Brozany | 3:0 | |
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| 29. Round | 01/06/2024 | FK Pardubice B | - | FK Kolín | 0:4 |
Czech Republic