| 1. Round | 10/08/2024 | Sokol Živanice | - | FK Kolín | 1:1 | ||||
| 3. Round | 25/08/2024 | Slovan Liberec B | - | FK Kolín | 3:2 |  |
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| 17. Round | 28/08/2024 | Sokol Brozany | - | FK Kolín | 3:0 | ||||
| 4. Round | 31/08/2024 | FK Kolín | - | FK Teplice B | 1:0 | ||||
| 5. Round | 07/09/2024 | FK Jablonec B | - | FK Kolín | 1:1 | ||||
| 7. Round | 22/09/2024 | FC Hradec Králové B | - | FK Kolín | 0:0 | |
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| 8. Round | 28/09/2024 | FK Kolín | - | FK Mlada Boleslav B | 3:3 | ||||
| 10. Round | 12/10/2024 | FK Kolín | - | SK Zápy | 0:1 | ||||
| 11. Round | 19/10/2024 | FK Pardubice B | - | FK Kolín | 7:0 | |
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| 14. Round | 09/11/2024 | FK Kolín | - | FK Baník Most-Souš | 0:1 | ||||
| 16. Round | 23/11/2024 | FK Kolín | - | Chlumec nad Cidlinou | 0:1 |
Czech Republic