| 15. Round | 01/03/2025 | Sigma Olomouc [Youth B] | - | FK Dukla Praha [Youth B] | 3:1 |  |
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| 16. Round | 08/03/2025 | FK Dukla Praha [Youth B] | - | FC Zbrojovka Brno [Youth B] | 2:1 | ||||
| 17. Round | 14/03/2025 | Banik Ostrava [Youth B] | - | FK Dukla Praha [Youth B] | 4:1 | ||||
| 18. Round | 22/03/2025 | FK Dukla Praha [Youth B] | - | Dynamo České Budějovice [Youth B] | 4:4 | ||||
| 21. Round | 12/04/2025 | FK Dukla Praha [Youth B] | - | Vysočina Jihlava [Youth B] | 4:1 | ||||
| 22. Round | 18/04/2025 | FK Mladá Boleslav [Youth B] | - | FK Dukla Praha [Youth B] | 3:4 |  |
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| 23. Round | 26/04/2025 | FK Dukla Praha [Youth B] | - | FK Pardubice [Youth B] | 0:4 | ||||
| 25. Round | 10/05/2025 | FK Dukla Praha [Youth B] | - | FK Jablonec [Youth B] | 5:1 | ||||
| 24. Round | 13/05/2025 | Slavia Praha [Youth B] | - | FK Dukla Praha [Youth B] | 3:2 | ||||
| 26. Round | 17/05/2025 | Slovan Liberec [Youth B] | - | FK Dukla Praha [Youth B] | 2:3 | |
Czech Republic