| 16. Round | 08/03/2025 | Vysočina Jihlava [Youth] | - | FC Zlín [Youth] | 1:2 | ||||
| 17. Round | 14/03/2025 | Sigma Olomouc [Youth] | - | Vysočina Jihlava [Youth] | 3:1 | ||||
| 18. Round | 22/03/2025 | Vysočina Jihlava [Youth] | - | FC Zbrojovka Brno [Youth] | 2:1 | ||||
| 19. Round | 29/03/2025 | Banik Ostrava [Youth] | - | Vysočina Jihlava [Youth] | 6:0 | ||||
| 20. Round | 05/04/2025 | Vysočina Jihlava [Youth] | - | Dynamo České Budějovice [Youth] | 3:2 |  |
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| 21. Round | 12/04/2025 | FK Dukla Praha [Youth] | - | Vysočina Jihlava [Youth] | 2:1 | |
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| 22. Round | 17/04/2025 | Vysočina Jihlava [Youth] | - | Viktoria Plzeň [Youth] | 2:4 | ||||
| 23. Round | 26/04/2025 | Vysočina Jihlava [Youth] | - | AC Sparta Praha [Youth] | 1:0 | ||||
| 24. Round | 03/05/2025 | FK Mladá Boleslav [Youth] | - | Vysočina Jihlava [Youth] | 3:1 | ||||
| 25. Round | 10/05/2025 | Vysočina Jihlava [Youth] | - | FK Pardubice [Youth] | 0:0 |
Czech Republic