| 6. Round | 21/09/2024 | AC Sparta Praha B [Women] | - | FC Zlín [Women] | 4:0 |  |
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| 7. Round | 28/09/2024 | FK Dukla Praha [Women] | - | AC Sparta Praha B [Women] | 1:4 | ||||
| 5. Round | 02/10/2024 | AC Sparta Praha B [Women] | - | FC Hradec Králové [Women] | 1:0 | ||||
| 8. Round | 05/10/2024 | AC Sparta Praha B [Women] | - | SK Líšeň [Women] | 7:2 | |
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| 9. Round | 12/10/2024 | Prague Raptors FC [Women] | - | AC Sparta Praha B [Women] | 1:6 | |
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| 10. Round | 19/10/2024 | FK Teplice [Women] | - | AC Sparta Praha B [Women] | 1:7 | |
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| 12. Round | 02/11/2024 | Baník Ostrava [Women] | - | AC Sparta Praha B [Women] | 0:1 |  |
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| 13. Round | 09/03/2025 | AC Sparta Praha B [Women] | - | Sigma Olomouc [Women] | 8:1 | ||||
| 17. Round | 12/04/2025 | SK Líšeň [Women] | - | AC Sparta Praha B [Women] | 0:3 | ||||
| 18. Round | 18/04/2025 | AC Sparta Praha B [Women] | - | Prague Raptors FC [Women] | 2:0 |
Slovakia