| 2. Round | 24/08/2024 | AC Sparta Praha [Women] | - | Lokomotiva Brno H.H. [Women] | 5:0 | ||||
| 8. Round | 13/10/2024 | 1. FC Slovácko [Women] | - | Lokomotiva Brno H.H. [Women] | 2:1 | ||||
| 4. Round | 20/10/2024 | FK Pardubice [Women] | - | Lokomotiva Brno H.H. [Women] | 1:1 | ||||
| 10. Round | 09/11/2024 | Slavia Praha [Women] | - | Lokomotiva Brno H.H. [Women] | 7:1 |
Czech Republic