| 14. Round | 09/11/2024 | 1. FC Slovácko [Youth] | - | Viktoria Plzeň [Youth] | 1:0 | ||||
| 15. Round | 16/11/2024 | Vysočina Jihlava [Youth] | - | 1. FC Slovácko [Youth] | 1:1 | ||||
| 18. Round | 01/04/2025 | Slavia Praha [Youth] | - | 1. FC Slovácko [Youth] | 2:0 | ||||
| 26. Round | 17/05/2025 | 1. FC Slovácko [Youth] | - | Banik Ostrava [Youth] | 0:4 | ||||
| 27. Round | 22/05/2025 | Dynamo České Budějovice [Youth] | - | 1. FC Slovácko [Youth] | 3:2 | ||||
| 28. Round | 31/05/2025 | 1. FC Slovácko [Youth] | - | FK Dukla Praha [Youth] | 2:5 |  |
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| 29. Round | 07/06/2025 | Viktoria Plzeň [Youth] | - | 1. FC Slovácko [Youth] | 3:1 | |
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| 30. Round | 11/06/2025 | 1. FC Slovácko [Youth] | - | Vysočina Jihlava [Youth] | 3:3 |
Czech Republic