| 2. Round | 18/08/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 2:0 | ||||
| 3. Round | 24/08/2024 | TJ Jiskra Domažlice | - | FK Dukla Praha B | 3:1 | ||||
| 4. Round | 01/09/2024 | FK Dukla Praha B | - | Povltavská FA | 1:4 | ||||
| 5. Round | 06/09/2024 | FC Chomutov | - | FK Dukla Praha B | 3:4 | ||||
| 7. Round | 20/09/2024 | FK Příbram | - | FK Dukla Praha B | 2:2 |  |
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| 10. Round | 13/10/2024 | FK Dukla Praha B | - | Bohemians Praha 1905 B | 4:0 | ||||
| 11. Round | 19/10/2024 | SK Petřín Plzeň | - | FK Dukla Praha B | 0:2 | ||||
| 12. Round | 27/10/2024 | FK Dukla Praha B | - | FK MAS Táborsko B | 2:3 | ||||
| 13. Round | 01/11/2024 | FC Písek | - | FK Dukla Praha B | 1:1 | ||||
| 14. Round | 09/11/2024 | Dynamo České Budějovice B | - | FK Dukla Praha B | 2:1 | ||||
| 16. Round | 23/11/2024 | Sokol Hostouň | - | FK Dukla Praha B | 2:5 |
Czech Republic