| 18. Round | 09/03/2025 | FK Dukla Praha B | - | FK Příbram | 2:3 | ||||
| 19. Round | 15/03/2025 | Slovan Velvary | - | FK Dukla Praha B | 0:1 | ||||
| 20. Round | 23/03/2025 | FK Dukla Praha B | - | FK Motorlet Praha | 5:0 | ||||
| 21. Round | 31/03/2025 | Bohemians Praha 1905 B | - | FK Dukla Praha B | 2:0 | ||||
| 22. Round | 06/04/2025 | FK Dukla Praha B | - | SK Petřín Plzeň | 3:1 | ||||
| 23. Round | 13/04/2025 | FK MAS Táborsko B | - | FK Dukla Praha B | 0:3 | ||||
| 24. Round | 19/04/2025 | FK Dukla Praha B | - | FC Písek | 0:2 | ||||
| 25. Round | 27/04/2025 | FK Dukla Praha B | - | Sokol Hostouň | 1:0 | ||||
| 26. Round | 04/05/2025 | Viktoria Plzeň B | - | FK Dukla Praha B | 1:1 | ||||
| 27. Round | 11/05/2025 | FK Dukla Praha B | - | FK Admira Praha | 6:0 |  |
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| 28. Round | 17/05/2025 | FK Loko Vltavín | - | FK Dukla Praha B | 1:0 |
Czech Republic