| 1. Round | 10/08/2024 | Banik Ostrava [Youth] | - | Dynamo České Budějovice [Youth] | 2:1 | ||||
| 2. Round | 17/08/2024 | FK Dukla Praha [Youth] | - | Banik Ostrava [Youth] | 0:1 |  |
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| 3. Round | 24/08/2024 | Banik Ostrava [Youth] | - | Viktoria Plzeň [Youth] | 1:0 | ||||
| 4. Round | 31/08/2024 | Vysočina Jihlava [Youth] | - | Banik Ostrava [Youth] | 1:3 | ||||
| 7. Round | 21/09/2024 | Banik Ostrava [Youth] | - | Slavia Praha [Youth] | 4:2 | |
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| 9. Round | 05/10/2024 | Banik Ostrava [Youth] | - | Slovan Liberec [Youth] | 7:1 | ||||
| 11. Round | 19/10/2024 | Banik Ostrava [Youth] | - | 1. FC Slovácko [Youth] | 0:1 | ||||
| 5. Round | 23/10/2024 | Banik Ostrava [Youth] | - | FK Mladá Boleslav [Youth] | 3:3 | |
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| 12. Round | 29/10/2024 | FC Zlín [Youth] | - | Banik Ostrava [Youth] | 0:4 | ||||
| 6. Round | 06/11/2024 | FK Pardubice [Youth] | - | Banik Ostrava [Youth] | 1:1 | ||||
| 14. Round | 09/11/2024 | FC Zbrojovka Brno [Youth] | - | Banik Ostrava [Youth] | 0:2 | ||||
| 10. Round | 20/11/2024 | SFC Opava [Youth] | - | Banik Ostrava [Youth] | 1:6 |  |
Czech Republic