| 12. Round | 05/10/2024 | Zbrojovka Brno | - | FC Sellier & Bellot Vlašim | 2:2 | ||||
| 13. Round | 20/10/2024 | Viktoria Žižkov | - | Zbrojovka Brno | 4:2 | ||||
| 15. Round | 03/11/2024 | Baník Ostrava B | - | Zbrojovka Brno | 1:2 | ||||
| 16. Round | 09/11/2024 | Zbrojovka Brno | - | SFC Opava | 2:2 | ||||
| 17. Round | 01/03/2025 | Zbrojovka Brno | - | MFK Chrudim | 0:0 | ||||
| 18. Round | 09/03/2025 | FK Varnsdorf | - | Zbrojovka Brno | 0:3 | ||||
| 19. Round | 15/03/2025 | Zbrojovka Brno | - | FK MAS Táborsko | 0:2 | ||||
| 20. Round | 30/03/2025 | AC Sparta Praha B | - | Zbrojovka Brno | 1:1 |  |
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| 30. Round | 25/05/2025 | Zbrojovka Brno | - | Vysočina Jihlava | 1:1 |
Czech Republic