| 16. Round | 08/03/2025 | FK Mladá Boleslav [Youth] | - | 1. FC Slovácko [Youth] | 5:0 | ||||
| 17. Round | 14/03/2025 | 1. FC Slovácko [Youth] | - | FK Pardubice [Youth] | 2:2 | ||||
| 19. Round | 29/03/2025 | 1. FC Slovácko [Youth] | - | FK Jablonec [Youth] | 1:2 |  |
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| 18. Round | 01/04/2025 | Slavia Praha [Youth] | - | 1. FC Slovácko [Youth] | 2:0 | ||||
| 20. Round | 05/04/2025 | Slovan Liberec [Youth] | - | 1. FC Slovácko [Youth] | 2:1 | ||||
| 21. Round | 12/04/2025 | 1. FC Slovácko [Youth] | - | SFC Opava [Youth] | 2:2 | ||||
| 22. Round | 18/04/2025 | AC Sparta Praha [Youth] | - | 1. FC Slovácko [Youth] | 2:0 | ||||
| 23. Round | 26/04/2025 | FC Zlín [Youth] | - | 1. FC Slovácko [Youth] | 1:0 | |
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| 26. Round | 17/05/2025 | 1. FC Slovácko [Youth] | - | Banik Ostrava [Youth] | 0:4 | ||||
| 27. Round | 22/05/2025 | Dynamo České Budějovice [Youth] | - | 1. FC Slovácko [Youth] | 3:2 | ||||
| 28. Round | 31/05/2025 | 1. FC Slovácko [Youth] | - | FK Dukla Praha [Youth] | 2:5 | ||||
| 29. Round | 07/06/2025 | Viktoria Plzeň [Youth] | - | 1. FC Slovácko [Youth] | 3:1 | ||||
| 30. Round | 11/06/2025 | 1. FC Slovácko [Youth] | - | Vysočina Jihlava [Youth] | 3:3 |
Czech Republic