| 18. Round | 17/03/2024 | Slavia Praha B | - | FK Loko Vltavín | 3:0 | ||||
| 19. Round | 23/03/2024 | FK Loko Vltavín | - | Sokol Hostouň | 1:0 | ||||
| 20. Round | 29/03/2024 | FK Admira Praha | - | FK Loko Vltavín | 1:2 |  |
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| 22. Round | 13/04/2024 | Dynamo České Budějovice B | - | FK Loko Vltavín | 2:1 | ||||
| 23. Round | 21/04/2024 | Povltavská FA | - | FK Loko Vltavín | 0:1 | ||||
| 24. Round | 27/04/2024 | FK Loko Vltavín | - | FK Robstav | 3:2 | ||||
| 25. Round | 05/05/2024 | Viktoria Plzeň B | - | FK Loko Vltavín | 3:1 | ||||
| 27. Round | 19/05/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 0:3 | ||||
| 28. Round | 25/05/2024 | FK Loko Vltavín | - | FK Motorlet Praha | 1:1 | ||||
| 29. Round | 02/06/2024 | Bohemians Praha 1905 B | - | FK Loko Vltavín | 1:3 | |
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| 30. Round | 08/06/2024 | FK Loko Vltavín | - | FC Písek | 5:1 |
Czech Republic