| 1. Round | 15/02/2025 | Júbilo Iwata | - | Mito HollyHock | 3:2 | ||||
| 2. Round | 23/02/2025 | Mito HollyHock | - | Montedio Yamagata | 1:0 | ||||
| 3. Round | 02/03/2025 | Mito HollyHock | - | Ehime FC | 1:1 | ||||
| 4. Round | 09/03/2025 | Oita Trinita | - | Mito HollyHock | 0:0 | ||||
| 5. Round | 16/03/2025 | Mito HollyHock | - | Vegalta Sendai | 2:2 |  |
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| 6. Round | 23/03/2025 | RB Omiya Ardija | - | Mito HollyHock | 2:0 | ||||
| 7. Round | 30/03/2025 | Mito HollyHock | - | Iwaki FC | 4:1 |  |
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| 8. Round | 05/04/2025 | JEF United Chiba | - | Mito HollyHock | 2:1 | ||||
| 9. Round | 12/04/2025 | Mito HollyHock | - | Hokkaido Consadole Sapporo | 3:1 | ||||
| 10. Round | 20/04/2025 | Tokushima Vortis | - | Mito HollyHock | 0:1 | ||||
| 11. Round | 25/04/2025 | Mito HollyHock | - | Ventforet Kofu | 0:0 | ||||
| 12. Round | 29/04/2025 | Mito HollyHock | - | FC Imabari | 0:0 | ||||
| 13. Round | 03/05/2025 | Fujieda MyFC | - | Mito HollyHock | 0:2 | |
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| 14. Round | 06/05/2025 | Renofa Yamaguchi | - | Mito HollyHock | 1:2 | ||||
| 15. Round | 11/05/2025 | Mito HollyHock | - | Blaublitz Akita | 2:1 | ||||
| 16. Round | 17/05/2025 | Mito HollyHock | - | Roasso Kumamoto | 2:1 |
Japan