| 3. Round | 24/08/2024 | TJ Jiskra Domažlice | - | FK Dukla Praha B | 3:1 | ||||
| 4. Round | 01/09/2024 | FK Dukla Praha B | - | Povltavská FA | 1:4 | ||||
| 7. Round | 20/09/2024 | FK Příbram | - | FK Dukla Praha B | 2:2 | ||||
| 8. Round | 29/09/2024 | FK Dukla Praha B | - | Slovan Velvary | 1:1 | ||||
| 9. Round | 05/10/2024 | FK Motorlet Praha | - | FK Dukla Praha B | 2:0 | ||||
| 10. Round | 13/10/2024 | FK Dukla Praha B | - | Bohemians Praha 1905 B | 4:0 | ||||
| 11. Round | 19/10/2024 | SK Petřín Plzeň | - | FK Dukla Praha B | 0:2 | ||||
| 18. Round | 09/03/2025 | FK Dukla Praha B | - | FK Příbram | 2:3 | ||||
| 19. Round | 15/03/2025 | Slovan Velvary | - | FK Dukla Praha B | 0:1 | ||||
| 22. Round | 06/04/2025 | FK Dukla Praha B | - | SK Petřín Plzeň | 3:1 | ||||
| 24. Round | 19/04/2025 | FK Dukla Praha B | - | FC Písek | 0:2 | ||||
| 25. Round | 27/04/2025 | FK Dukla Praha B | - | Sokol Hostouň | 1:0 | ||||
| 27. Round | 11/05/2025 | FK Dukla Praha B | - | FK Admira Praha | 6:0 | ||||
| 28. Round | 17/05/2025 | FK Loko Vltavín | - | FK Dukla Praha B | 1:0 | ||||
| 29. Round | 25/05/2025 | FK Dukla Praha B | - | TJ Jiskra Domažlice | 0:5 | ||||
| 30. Round | 01/06/2025 | Povltavská FA | - | FK Dukla Praha B | 1:4 |  |
Czech Republic