| 1. Round | 04/08/2023 | MFK Karviná [Youth B] | - | FC Zbrojovka Brno [Youth B] | 1:6 | ||||
| 2. Round | 11/08/2023 | FC Zbrojovka Brno [Youth B] | - | Viktoria Plzeň [Youth B] | 3:2 | ||||
| 3. Round | 19/08/2023 | 1. FC Slovácko [Youth B] | - | FC Zbrojovka Brno [Youth B] | 1:1 |  |
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| 4. Round | 25/08/2023 | FC Zbrojovka Brno [Youth B] | - | Sigma Olomouc [Youth B] | 3:1 | ||||
| 5. Round | 02/09/2023 | FK Pardubice [Youth B] | - | FC Zbrojovka Brno [Youth B] | 6:2 | ||||
| 6. Round | 09/09/2023 | FC Zbrojovka Brno [Youth B] | - | FC Zlín [Youth B] | 0:0 | ||||
| 7. Round | 16/09/2023 | Dynamo České Budějovice [Youth B] | - | FC Zbrojovka Brno [Youth B] | 2:2 | ||||
| 8. Round | 23/09/2023 | FC Zbrojovka Brno [Youth B] | - | Vysočina Jihlava [Youth B] | 3:2 |
Czech Republic