| 17. Round | 02/03/2025 | Slavia Praha B | - | SK Líšeň | 3:0 | ||||
| 20. Round | 30/03/2025 | Sigma Olomouc B | - | Slavia Praha B | 2:2 | ||||
| 21. Round | 06/04/2025 | Slavia Praha B | - | FC Zlín | 0:1 | ||||
| 22. Round | 11/04/2025 | 1. SK Prostějov | - | Slavia Praha B | 0:0 | ||||
| 23. Round | 16/04/2025 | Slavia Praha B | - | FC Sellier & Bellot Vlašim | 1:1 | ||||
| 24. Round | 20/04/2025 | Viktoria Žižkov | - | Slavia Praha B | 4:2 |  |
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| 26. Round | 04/05/2025 | Slavia Praha B | - | SFC Opava | 0:1 | |
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| 27. Round | 07/05/2025 | Baník Ostrava B | - | Slavia Praha B | 0:3 | ||||
| 28. Round | 11/05/2025 | Slavia Praha B | - | Zbrojovka Brno | 1:1 | ||||
| 30. Round | 25/05/2025 | Slavia Praha B | - | FK MAS Táborsko | 1:0 |  |
Czech Republic