| 1. Round | 21/07/2024 | FK Varnsdorf | - | Viktoria Žižkov | 1:1 | ||||
| 3. Round | 31/07/2024 | FK Varnsdorf | - | SFC Opava | 3:1 | ||||
| 4. Round | 03/08/2024 | Zbrojovka Brno | - | FK Varnsdorf | 1:6 |  |
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| 2. Round | 07/08/2024 | Baník Ostrava B | - | FK Varnsdorf | 2:0 | ||||
| 5. Round | 11/08/2024 | AC Sparta Praha B | - | FK Varnsdorf | 1:1 | ||||
| 7. Round | 24/08/2024 | SK Líšeň | - | FK Varnsdorf | 1:0 | ||||
| 8. Round | 01/09/2024 | FK Varnsdorf | - | MFK Vyškov | 1:2 | |
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| 6. Round | 18/09/2024 | FK Varnsdorf | - | MFK Chrudim | 0:3 | ||||
| 10. Round | 22/09/2024 | FK Varnsdorf | - | Sigma Olomouc B | 2:3 | ||||
| 11. Round | 28/09/2024 | 1. SK Prostějov | - | FK Varnsdorf | 1:1 | ||||
| 9. Round | 02/10/2024 | FK MAS Táborsko | - | FK Varnsdorf | 2:0 | ||||
| 12. Round | 06/10/2024 | FK Varnsdorf | - | FC Zlín | 0:2 | ||||
| 13. Round | 18/10/2024 | Vysočina Jihlava | - | FK Varnsdorf | 1:1 | ||||
| 14. Round | 27/10/2024 | FK Varnsdorf | - | FC Sellier & Bellot Vlašim | 1:2 | ||||
| 15. Round | 03/11/2024 | Slavia Praha B | - | FK Varnsdorf | 2:1 | ||||
| 16. Round | 10/11/2024 | FK Varnsdorf | - | Baník Ostrava B | 0:0 |
Czech Republic