| 5. Round | 12/08/2023 | Zbrojovka Brno | - | MFK Chrudim | 4:1 | ||||
| 6. Round | 18/08/2023 | AC Sparta Praha B | - | Zbrojovka Brno | 1:2 |  |
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| 7. Round | 26/08/2023 | 1. SK Prostějov | - | Zbrojovka Brno | 0:1 | |
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| 8. Round | 02/09/2023 | Zbrojovka Brno | - | FK Varnsdorf | 1:0 | |
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| 9. Round | 15/09/2023 | Vysočina Jihlava | - | Zbrojovka Brno | 0:1 | ||||
| 10. Round | 23/09/2023 | Zbrojovka Brno | - | MFK Vyškov | 0:1 | ||||
| 20. Round | 29/03/2024 | Zbrojovka Brno | - | AC Sparta Praha B | 2:3 | |
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| 22. Round | 14/04/2024 | FK Varnsdorf | - | Zbrojovka Brno | 4:1 | |
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| 23. Round | 17/04/2024 | Zbrojovka Brno | - | Vysočina Jihlava | 1:0 | ||||
| 25. Round | 27/04/2024 | Zbrojovka Brno | - | SFC Opava | 0:1 | |
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| 26. Round | 03/05/2024 | FK Příbram | - | Zbrojovka Brno | 0:2 | ||||
| 30. Round | 25/05/2024 | FC Sellier & Bellot Vlašim | - | Zbrojovka Brno | 2:2 | |
Czech Republic