| 1. Round | 22/07/2024 | Vysočina Jihlava | - | Zbrojovka Brno | 0:2 | ||||
| 2. Round | 26/07/2024 | SFC Opava | - | Zbrojovka Brno | 1:1 |  |
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| 3. Round | 31/07/2024 | MFK Chrudim | - | Zbrojovka Brno | 1:0 | ||||
| 4. Round | 03/08/2024 | Zbrojovka Brno | - | FK Varnsdorf | 1:6 |  |
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| 8. Round | 01/09/2024 | Zbrojovka Brno | - | FC Zlín | 1:2 | ||||
| 11. Round | 29/09/2024 | Sigma Olomouc B | - | Zbrojovka Brno | 4:1 | |
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| 15. Round | 03/11/2024 | Baník Ostrava B | - | Zbrojovka Brno | 1:2 | ||||
| 16. Round | 09/11/2024 | Zbrojovka Brno | - | SFC Opava | 2:2 | |
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| 17. Round | 01/03/2025 | Zbrojovka Brno | - | MFK Chrudim | 0:0 | ||||
| 20. Round | 30/03/2025 | AC Sparta Praha B | - | Zbrojovka Brno | 1:1 | ||||
| 24. Round | 19/04/2025 | SK Líšeň | - | Zbrojovka Brno | 1:4 | |
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| 29. Round | 17/05/2025 | Zbrojovka Brno | - | Baník Ostrava B | 1:0 | |
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| 30. Round | 25/05/2025 | Zbrojovka Brno | - | Vysočina Jihlava | 1:1 |
Czech Republic