| 1. Round | 24/07/2009 | FK Teplice | - | AC Sparta Praha | 0:0 | ||||
| 7. Round | 12/09/2009 | AC Sparta Praha | - | FK Baumit Jablonec | 2:0 | ||||
| 8. Round | 21/09/2009 | 1. FC Brno | - | AC Sparta Praha | 1:1 |  |
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| 9. Round | 26/09/2009 | AC Sparta Praha | - | Viktoria Plzeň | 0:0 | ||||
| 10. Round | 05/10/2009 | Slavia Praha | - | AC Sparta Praha | 0:1 |  |
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| 11. Round | 17/10/2009 | AC Sparta Praha | - | Dynamo České Budějovice | 1:1 | ||||
| 12. Round | 25/10/2009 | Bohemians Praha | - | AC Sparta Praha | 2:3 | ||||
| 13. Round | 31/10/2009 | AC Sparta Praha | - | Baník Ostrava | 1:1 | ||||
| 14. Round | 09/11/2009 | 1. FC Slovácko | - | AC Sparta Praha | 0:1 | |
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| 15. Round | 21/11/2009 | AC Sparta Praha | - | Slovan Liberec | 2:0 | |
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| 16. Round | 28/11/2009 | Bohemians 1905 | - | AC Sparta Praha | 0:2 | |
Czech Republic