| 2. Round | 18/08/2024 | FK Dukla Praha B | - | FK Loko Vltavín | 2:0 | ||||
| 17. Round | 28/08/2024 | FK Dukla Praha B | - | Viktoria Plzeň B | 2:0 | ||||
| 5. Round | 06/09/2024 | FC Chomutov | - | FK Dukla Praha B | 3:4 |  |
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| 8. Round | 29/09/2024 | FK Dukla Praha B | - | Slovan Velvary | 1:1 | ||||
| 16. Round | 23/11/2024 | Sokol Hostouň | - | FK Dukla Praha B | 2:5 | ||||
| 18. Round | 09/03/2025 | FK Dukla Praha B | - | FK Příbram | 2:3 | ||||
| 23. Round | 13/04/2025 | FK MAS Táborsko B | - | FK Dukla Praha B | 0:3 | ||||
| 26. Round | 04/05/2025 | Viktoria Plzeň B | - | FK Dukla Praha B | 1:1 | ||||
| 32. Round | 07/05/2025 | FK Dukla Praha B | - | Dynamo České Budějovice B | 0:2 |
Czech Republic