| 1. Round | 20/07/2024 | FK MAS Táborsko | - | Slavia Praha B | 3:1 |  |
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| 2. Round | 28/07/2024 | Slavia Praha B | - | AC Sparta Praha B | 2:1 | ||||
| 3. Round | 31/07/2024 | SK Líšeň | - | Slavia Praha B | 0:4 | |
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| 4. Round | 04/08/2024 | Slavia Praha B | - | MFK Chrudim | 0:1 | ||||
| 5. Round | 12/08/2024 | MFK Vyškov | - | Slavia Praha B | 1:0 | ||||
| 6. Round | 18/08/2024 | Slavia Praha B | - | Sigma Olomouc B | 4:0 | |
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| 8. Round | 01/09/2024 | Slavia Praha B | - | 1. SK Prostějov | 2:2 | |
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| 7. Round | 17/09/2024 | FC Zlín | - | Slavia Praha B | 2:1 | ||||
| 10. Round | 22/09/2024 | Slavia Praha B | - | Viktoria Žižkov | 2:2 | |
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| 11. Round | 29/09/2024 | Slavia Praha B | - | Vysočina Jihlava | 2:0 | ||||
| 9. Round | 02/10/2024 | FC Sellier & Bellot Vlašim | - | Slavia Praha B | 2:2 | |
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| 12. Round | 06/10/2024 | SFC Opava | - | Slavia Praha B | 2:1 | ||||
| 13. Round | 20/10/2024 | Slavia Praha B | - | Baník Ostrava B | 1:3 | |
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| 15. Round | 03/11/2024 | Slavia Praha B | - | FK Varnsdorf | 2:1 | ||||
| 16. Round | 10/11/2024 | AC Sparta Praha B | - | Slavia Praha B | 1:1 | |
Czech Republic