| 6. Round | 08/09/2000 | FK Drnovice | - | Baník Ostrava | 3:0 | ||||
| 7. Round | 16/09/2000 | AC Sparta Praha | - | FK Drnovice | 3:0 | ||||
| 8. Round | 22/09/2000 | FK Drnovice | - | FK Teplice | 3:1 | ||||
| 9. Round | 29/09/2000 | Sigma Olomouc | - | FK Drnovice | 2:0 |  |
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| 10. Round | 15/10/2000 | FK Drnovice | - | FC Marila Příbram | 0:1 | ||||
| 11. Round | 22/10/2000 | FC Stavo Artikel Brno | - | FK Drnovice | 0:0 | |
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| 13. Round | 05/11/2000 | FK Jablonec 97 | - | FK Drnovice | 0:0 | |
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| 14. Round | 12/11/2000 | FK Drnovice | - | Dynamo České Budějovice | 2:0 | ||||
| 15. Round | 18/11/2000 | Slavia Praha | - | FK Drnovice | 0:0 | ||||
| 16. Round | 26/11/2000 | Chmel Blsany | - | FK Drnovice | 0:0 |  |
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| 17. Round | 18/02/2001 | 1. FC Synot Stare Mesto | - | FK Drnovice | 1:2 | |
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| 18. Round | 04/03/2001 | CU Bohemians Praha | - | FK Drnovice | 0:3 | ||||
| 19. Round | 11/03/2001 | FK Drnovice | - | Slovan Liberec | 2:1 | ||||
| 20. Round | 17/03/2001 | Baník Ostrava | - | FK Drnovice | 2:0 | |
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| 23. Round | 08/04/2001 | FK Teplice | - | FK Drnovice | 0:1 | ||||
| 24. Round | 15/04/2001 | FK Drnovice | - | Sigma Olomouc | 1:2 |
Czech Republic