| 1. Round | 03/08/2024 | TJ Unie Hlubina | - | FC Slovan Rosice | 4:0 | ||||
| 2. Round | 10/08/2024 | FC Slovan Rosice | - | FK Třinec | 0:1 | ||||
| 3. Round | 17/08/2024 | FK Hodonín | - | FC Slovan Rosice | 2:2 | ||||
| 4. Round | 24/08/2024 | FC Slovan Rosice | - | 1. FC Slovácko B | 3:1 | ||||
| 5. Round | 01/09/2024 | FK Frýdek-Místek | - | FC Slovan Rosice | 1:4 | ||||
| 6. Round | 06/09/2024 | FC Slovan Rosice | - | 1. SC Znojmo | 2:1 | ||||
| 17. Round | 10/09/2024 | FC Slovan Rosice | - | Hanácká Slávia Kroměříž | 3:3 | ||||
| 8. Round | 20/09/2024 | FC Hlučín | - | FC Slovan Rosice | 2:0 | ||||
| 9. Round | 28/09/2024 | FC Slovan Rosice | - | MFK Karviná B | 6:3 | ||||
| 7. Round | 01/10/2024 | FC Slovan Rosice | - | TJ Start Brno | 0:0 | ||||
| 10. Round | 05/10/2024 | FC Zlín B | - | FC Slovan Rosice | 3:1 | ||||
| 11. Round | 12/10/2024 | FC Slovan Rosice | - | FC Zbrojovka Brno B | 1:2 | ||||
| 13. Round | 26/10/2024 | FC Slovan Rosice | - | ČSK Uherský Brod | 2:4 |
Czech Republic