| 4. Round | 23/08/2024 | TJ Start Brno | - | FK Hodonín | 2:2 | ||||
| 8. Round | 22/09/2024 | 1. SC Znojmo | - | TJ Start Brno | 2:3 | ||||
| 13. Round | 27/10/2024 | TJ Start Brno | - | FK Blansko | 2:2 | ||||
| 15. Round | 10/11/2024 | TJ Start Brno | - | FC Zlínsko | 1:1 | ||||
| 18. Round | 09/03/2025 | TJ Start Brno | - | Hanácká Slávia Kroměříž | 0:1 | ||||
| 22. Round | 05/04/2025 | TJ Start Brno | - | 1. FC Slovácko B | 0:1 | ||||
| 23. Round | 12/04/2025 | FK Frýdek-Místek | - | TJ Start Brno | 3:2 |  |
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| 24. Round | 17/04/2025 | TJ Start Brno | - | FC Slovan Rosice | 4:1 | ||||
| 25. Round | 25/04/2025 | TJ Start Brno | - | 1. SC Znojmo | 3:1 | ||||
| 33. Round | 30/04/2025 | TJ Start Brno | - | SK Uničov | 0:3 | ||||
| 26. Round | 03/05/2025 | FC Hlučín | - | TJ Start Brno | 1:3 | ||||
| 28. Round | 17/05/2025 | FC Zlín B | - | TJ Start Brno | 2:3 | ||||
| 29. Round | 24/05/2025 | TJ Start Brno | - | FC Zbrojovka Brno B | 1:1 | |
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| 30. Round | 30/05/2025 | FK Blansko | - | TJ Start Brno | 3:0 |
Czech Republic