| 1. Round | 29/07/2000 | 1. FC Synot Stare Mesto | - | Slavia Praha | 1:1 | ||||
| 9. Round | 01/10/2000 | FC Marila Příbram | - | Slavia Praha | 2:1 | ||||
| 10. Round | 14/10/2000 | Slavia Praha | - | FC Stavo Artikel Brno | 2:1 |  |
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| 13. Round | 04/11/2000 | Dynamo České Budějovice | - | Slavia Praha | 3:2 | |
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| 14. Round | 12/11/2000 | Chmel Blsany | - | Slavia Praha | 2:1 | |
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| 15. Round | 18/11/2000 | Slavia Praha | - | FK Drnovice | 0:0 | |
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| 6. Round | 01/12/2000 | Slavia Praha | - | AC Sparta Praha | 1:3 | ||||
| 8. Round | 11/12/2000 | Slavia Praha | - | Sigma Olomouc | 2:2 | ||||
| 21. Round | 31/03/2001 | Slavia Praha | - | FK Teplice | 3:0 | |
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| 22. Round | 03/04/2001 | Slavia Praha | - | CU Bohemians Praha | 1:0 |  |
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| 24. Round | 13/04/2001 | Slavia Praha | - | FC Marila Příbram | 0:0 | ||||
| 25. Round | 21/04/2001 | FC Stavo Artikel Brno | - | Slavia Praha | 0:2 | ||||
| 26. Round | 28/04/2001 | Slavia Praha | - | Viktoria Žižkov | 1:0 | |
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| 27. Round | 06/05/2001 | FK Jablonec 97 | - | Slavia Praha | 1:2 | ||||
| 28. Round | 14/05/2001 | Slavia Praha | - | Dynamo České Budějovice | 1:0 |
Czech Republic