| 1. Round | 10/08/2024 | SK Kladno | - | FK Teplice B | 7:3 |  |
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| 2. Round | 17/08/2024 | Jiskra Ústí nad Orlicí | - | SK Kladno | 0:1 | ||||
| 3. Round | 24/08/2024 | SK Kladno | - | FK Mlada Boleslav B | 2:0 | ||||
| 4. Round | 01/09/2024 | SK Zápy | - | SK Kladno | 2:2 | |
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| 5. Round | 06/09/2024 | SK Kladno | - | FK Ústí nad Labem | 2:1 | |
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| 6. Round | 14/09/2024 | FK Baník Most-Souš | - | SK Kladno | 3:0 | ||||
| 7. Round | 21/09/2024 | SK Kladno | - | Chlumec nad Cidlinou | 3:2 | ||||
| 8. Round | 28/09/2024 | Sokol Živanice | - | SK Kladno | 1:0 | ||||
| 9. Round | 05/10/2024 | SK Kladno | - | Slovan Liberec B | 4:0 | |
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| 10. Round | 13/10/2024 | FK Jablonec B | - | SK Kladno | 0:4 | |
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| 11. Round | 19/10/2024 | SK Kladno | - | FC Hradec Králové B | 3:0 | |
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| 12. Round | 26/10/2024 | Arsenal Česká Lípa | - | SK Kladno | 0:1 | |
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| 13. Round | 02/11/2024 | SK Kladno | - | FK Pardubice B | 1:0 | ||||
| 14. Round | 10/11/2024 | Benátky nad Jizerou | - | SK Kladno | 1:1 | ||||
| 15. Round | 16/11/2024 | SK Kladno | - | FK Kolín | 0:2 | ||||
| 16. Round | 23/11/2024 | SK Kladno | - | Sokol Brozany | 1:3 |
Czech Republic