| 1. Round | 30/07/2000 | Viktoria Plzeň | - | Dynamo České Budějovice | 2:2 | ||||
| 2. Round | 05/08/2000 | Slavia Praha | - | Viktoria Plzeň | 2:1 | ||||
| 3. Round | 13/08/2000 | Viktoria Plzeň | - | FK Drnovice | 2:1 | ||||
| 4. Round | 18/08/2000 | 1. FC Synot Stare Mesto | - | Viktoria Plzeň | 5:0 | ||||
| 5. Round | 27/08/2000 | Viktoria Plzeň | - | Chmel Blsany | 1:1 | ||||
| 6. Round | 10/09/2000 | Viktoria Plzeň | - | CU Bohemians Praha | 1:1 | ||||
| 7. Round | 17/09/2000 | Slovan Liberec | - | Viktoria Plzeň | 2:0 | ||||
| 8. Round | 24/09/2000 | Viktoria Plzeň | - | Baník Ostrava | 1:1 | ||||
| 9. Round | 01/10/2000 | AC Sparta Praha | - | Viktoria Plzeň | 5:1 | ||||
| 10. Round | 15/10/2000 | Viktoria Plzeň | - | FK Teplice | 0:2 | ||||
| 11. Round | 22/10/2000 | Sigma Olomouc | - | Viktoria Plzeň | 4:0 |  |
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| 12. Round | 29/10/2000 | Viktoria Plzeň | - | FC Marila Příbram | 1:1 | ||||
| 13. Round | 05/11/2000 | FC Stavo Artikel Brno | - | Viktoria Plzeň | 1:0 | ||||
| 14. Round | 12/11/2000 | Viktoria Plzeň | - | Viktoria Žižkov | 0:0 | ||||
| 15. Round | 19/11/2000 | FK Jablonec 97 | - | Viktoria Plzeň | 2:0 | ||||
| 16. Round | 26/11/2000 | Dynamo České Budějovice | - | Viktoria Plzeň | 4:3 |
Czech Republic