| 3. Round | 31/08/2024 | FK Dukla Praha [Women] | - | FK Teplice [Women] | 1:2 | ||||
| 4. Round | 08/09/2024 | Dynamo České Budějovice [Women] | - | FK Dukla Praha [Women] | 3:0 | ||||
| 6. Round | 21/09/2024 | Sigma Olomouc [Women] | - | FK Dukla Praha [Women] | 6:0 | ||||
| 7. Round | 28/09/2024 | FK Dukla Praha [Women] | - | AC Sparta Praha B [Women] | 1:4 | ||||
| 8. Round | 06/10/2024 | FC Zlín [Women] | - | FK Dukla Praha [Women] | 0:2 | ||||
| 9. Round | 12/10/2024 | FK Dukla Praha [Women] | - | FC Hradec Králové [Women] | 1:2 | ||||
| 10. Round | 19/10/2024 | SK Líšeň [Women] | - | FK Dukla Praha [Women] | 1:5 |  |
|||
| 11. Round | 26/10/2024 | FK Dukla Praha [Women] | - | Prague Raptors FC [Women] | 1:4 | ||||
| 12. Round | 02/11/2024 | FK Teplice [Women] | - | FK Dukla Praha [Women] | 2:2 | ||||
| 5. Round | 09/11/2024 | FK Dukla Praha [Women] | - | Baník Ostrava [Women] | 0:1 |
Czech Republic