| 1. Round | 17/08/2024 | FK Dukla Praha [Women] | - | SK Líšeň [Women] | 2:2 | ||||
| 2. Round | 24/08/2024 | Prague Raptors FC [Women] | - | FK Dukla Praha [Women] | 1:2 | ||||
| 3. Round | 31/08/2024 | FK Dukla Praha [Women] | - | FK Teplice [Women] | 1:2 | ||||
| 9. Round | 12/10/2024 | FK Dukla Praha [Women] | - | FC Hradec Králové [Women] | 1:2 | ||||
| 10. Round | 19/10/2024 | SK Líšeň [Women] | - | FK Dukla Praha [Women] | 1:5 | ||||
| 11. Round | 26/10/2024 | FK Dukla Praha [Women] | - | Prague Raptors FC [Women] | 1:4 | ||||
| 12. Round | 02/11/2024 | FK Teplice [Women] | - | FK Dukla Praha [Women] | 2:2 | ||||
| 15. Round | 23/03/2025 | FK Dukla Praha [Women] | - | Sigma Olomouc [Women] | 2:3 |  |
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| 16. Round | 30/03/2025 | AC Sparta Praha B [Women] | - | FK Dukla Praha [Women] | 7:0 | ||||
| 17. Round | 13/04/2025 | FK Dukla Praha [Women] | - | FC Zlín [Women] | 2:3 | |
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| 18. Round | 19/04/2025 | FC Hradec Králové [Women] | - | FK Dukla Praha [Women] | 2:0 |
Czech Republic