| 1. Round | 11/08/2024 | FK Admira Praha | - | FK Dukla Praha B | 1:0 | ||||
| 3. Round | 24/08/2024 | TJ Jiskra Domažlice | - | FK Dukla Praha B | 3:1 | ||||
| 17. Round | 28/08/2024 | FK Dukla Praha B | - | Viktoria Plzeň B | 2:0 | ||||
| 4. Round | 01/09/2024 | FK Dukla Praha B | - | Povltavská FA | 1:4 | ||||
| 5. Round | 06/09/2024 | FC Chomutov | - | FK Dukla Praha B | 3:4 | ||||
| 7. Round | 20/09/2024 | FK Příbram | - | FK Dukla Praha B | 2:2 | ||||
| 9. Round | 05/10/2024 | FK Motorlet Praha | - | FK Dukla Praha B | 2:0 | ||||
| 10. Round | 13/10/2024 | FK Dukla Praha B | - | Bohemians Praha 1905 B | 4:0 | ||||
| 11. Round | 19/10/2024 | SK Petřín Plzeň | - | FK Dukla Praha B | 0:2 | ||||
| 12. Round | 27/10/2024 | FK Dukla Praha B | - | FK MAS Táborsko B | 2:3 | ||||
| 14. Round | 09/11/2024 | Dynamo České Budějovice B | - | FK Dukla Praha B | 2:1 | ||||
| 18. Round | 09/03/2025 | FK Dukla Praha B | - | FK Příbram | 2:3 |  |
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| 19. Round | 15/03/2025 | Slovan Velvary | - | FK Dukla Praha B | 0:1 | ||||
| 20. Round | 23/03/2025 | FK Dukla Praha B | - | FK Motorlet Praha | 5:0 |
Czech Republic