| 1. Round | 25/07/2009 | 1. FC Slovácko | - | Viktoria Plzeň | 2:3 | ||||
| 2. Round | 03/08/2009 | FK Baumit Jablonec | - | 1. FC Slovácko | 3:2 | ||||
| 3. Round | 09/08/2009 | 1. FC Slovácko | - | Sigma Olomouc | 2:0 |  |
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| 4. Round | 14/08/2009 | 1. FC Brno | - | 1. FC Slovácko | 1:0 | ||||
| 17. Round | 28/02/2010 | Sigma Olomouc | - | 1. FC Slovácko | 1:0 | ||||
| 18. Round | 06/03/2010 | 1. FC Slovácko | - | 1. FC Brno | 0:2 | ||||
| 19. Round | 14/03/2010 | Bohemians Praha | - | 1. FC Slovácko | 1:1 |  |
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| 20. Round | 22/03/2010 | 1. FC Slovácko | - | Slavia Praha | 3:1 | |
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| 21. Round | 25/03/2010 | FK Teplice | - | 1. FC Slovácko | 2:1 | |
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| 22. Round | 28/03/2010 | 1. FC Slovácko | - | Dynamo České Budějovice | 1:0 | ||||
| 23. Round | 03/04/2010 | 1. FC Slovácko | - | Slovan Liberec | 0:0 | ||||
| 24. Round | 10/04/2010 | Baník Ostrava | - | 1. FC Slovácko | 3:2 | |
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| 25. Round | 17/04/2010 | 1. FC Slovácko | - | Bohemians 1905 | 1:1 | ||||
| 26. Round | 25/04/2010 | SK Kladno | - | 1. FC Slovácko | 1:0 | ||||
| 27. Round | 30/04/2010 | 1. FC Slovácko | - | FK Mladá Boleslav | 1:0 | |
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| 29. Round | 08/05/2010 | 1. FC Slovácko | - | 1. FK Příbram | 2:1 | ||||
| 30. Round | 15/05/2010 | Viktoria Plzeň | - | 1. FC Slovácko | 2:2 |
Czech Republic